Fibrosis is defined as the excessive accumulation of extracellular matrix (ECM) components such as collagen and fibronectin in and around damaged and inflamed tissue 1 . It leads to tissue hardening, overgrowth, and scarring as a consequence of excessive extracellular matrix deposition.
Fibrosis is a common feature of pathologies resulting from chronic inflammation. Unlike acute inflammation, which is rapidly resolved, chronic inflammation persists for several months. Chronic inflammatory reactions are induced by a various stimuli including infections, allergic responses, chemical insults, and tissue injury. Although these process are initially beneficial, the prolonged inflammatory triggers the simultaneous activation of Inflammatory, remodelling, and repair processes
The effect on fibrosis of all the drugs was observed for both he acute
incubation experimental setup and chronic incubation individual setup. Though individually all the drugs were able to reduce the fibrotic properties the highest effect was observed for GC on all the time points and concentrations. With the treatment the renal fibrotic properties went down up to 30 percent which is particularly good effect given the rigidity of fibrotic cells.
The most significant effect was observed with the combination of Zerilox, GC and Vindia (35.40% maximum and 19.07% minimum. The exerted effect is of high importance because the fibrotic properties started to reduce even at exceptionally low concentration of 5ug/ml and totally followed the pattern of concentration and incubation time dependency. Overall, the experimental data indicates that given drugs are very potent candidates to be used in anti-renal fibrotic medicine and their effect should be further analyzed on multiple fibrosis causing genes.